OBJECTIVE: The objective of the study was to study the micro-flora and antibiotic pattern of the bacteria causing middle ear infection in order to provide a guideline for empirical antibiotic therapy. MATERIALS & METHODS: This is a cross sectional study performed in 100 cases of middle ear infection attending the outpatient department of ENT ( CAIMS ,Karimnagar ) from September 2014 to November 2014.Patients of clinically suspected middle ear infections are included in this study. Specimens were collected under sterile precautions and processed in microbiology department. RESULTS: Among the 100 samples, there were 56 isolates. Pseudomonas aeruginosa 33 (58.92%) was found to be a predominant pathogens followed by Staphylococci aureus 15(26.78%), Klebsiella pneumoniae 3(5.35%), CONS 2(3.57%), Proteus 2(3.57%) and Escherichia coli 1(1.78%). Among them 8 specimens were polymicrobial (14.28%). The antibiogram revealed Pseudomonas aeruginosa is multiple drug resistant. Out of 33 Pseudomonas aeruginosa 6 were ESBL and 2 were MBL producers .Among the 15 isolates of Staphylococci aureus 3 were found to be MRSA. Among the 5 Klebsiella pneumoniae 1 is ESBL producer. CONCLUSION : Because of the variation in climate, community and inadvertent use of antibiotics , the pattern of bacterial flora vary in middle ear infection .So it is useful and helpful to identify the microorganism as it gives us the clue regarding the use of correct antibiotics.