Intrinsic susceptibility of uremia rbc for hemolysis

Abstract

Using in vitro system; the compounds sodium dodecyl sulphate , nitrosocysteine, nitrosoarginine, cysteine and salicylic acid caused more than 60% hemolysis of RBC from uremia patients. This hemolysis was concentration dependent and showed more severity on patients with chronic kidney disease than on dialysis group. The IC50 values of hemolytic in RBC of uremia patients were significantly lower (p < 0.05) than their corresponding values in normal controls. Dialysis managed to decrease the potency of RBC hemolysis when compared with patients having chronic kidney disease. All these hemolytic changes were associated with a defect in total antioxidant capacities of uremia groups. However, some radical scavenger agents like trolox, ascorbic acid and uric acid were partially able to protect the uremia RBC from hemolysis. The current in vitro system has the potential to assess the susceptibility of uremia RBC for hemolysis, and may help to evaluate the degree of RBC exposure to harmful endotoxins or oxidative agents.