Expression of cytokeratin – 7 and cytokeratin – 19 inmice model biliary atresia

Abstract

Biliary atresia (BA) is a progressive inflammatory obstruction and fibro- obliteration of the partial or total extrahepatic and intrahepatic bile ducts during the perinatal period, with the etiology remains uncertain. In addition to damage caused by an immunological response, bile duct epithelial cells is thought to occur changes in the characteristics of epithelial cells into mesenchymal cell characteristics, that known as Epithelial Mesenchymal Transition (EMT).In the process of EMT, expression of cytokeratin – 7 (CK-7), cytokeratin – 19 (CK-19)is decreased. Objective: to investigate the changes of CK-7 and CK-19 expression in mice models Biliary Atresia. MethodTwo groups of neonatal mice, those were trial group consisted of neonatal mice injected by RRV 1.5 x 106 PFU intraperitoneally less than 24 hours after birth, and control group consisted of neonatal mice injected with buffered saline were used as BA model. The expressions of CK-7 and CK-19 of the two groupsof neonatal mice were studied on day 3rd, 7th, 14th, and 21th after birth by flowcytometry examination. Results:Median value of CK-7 and CK-19expressionsintrial group on day 3rd, 7th, 14th, and 21thsignificantly lower than control group(p<0.05). The difference in the value of CK-7 expression between trial group and control group progressively seen from day7th and the large difference in expression values ​obtained on day 21st. Expression of CK-19of trial group increased after decreased on day 3rdand continued until day 21st although it remained below the pattern control group. Conclusion: The greatest decreased of CK-7 expression in biliary atresia mice model was ​obtained on day 21stafter induction of RRV, while the expression of CK-19 still increased.