Cloning phbcaboperon of alcaligeneseutrophus h16 into escherichia coli dh5α to manufacture poly 3-hydroxybutyrate using molasses as carbon source

Abstract

In this study,a 4985 bp DNA fragment including the whole phbCAB operon from Alcaligeneseutrophus H16 was clonedinto Escherichia coli DH5α. This fragment contains three genes phbC, phbA, phbB encoding three enzymes P(3HB) polymerase, β-ketoacyl CoA thiolase and acetoacetyl-CoA dehydrogenase, respectively, which participate in PHB biosynthesis. This recombinant E. coli DH5α was performed batch fermentation in molasses pretreated with acid, 2.5g/l cell dried weight (CDW) and 22.5% PHB accumulation were obtained. This is the basis for future studies of optimization of PHB biosynthesis by recombinant bacteria using molasses ascarbon source.