Myocardial infarction (MI) or acute myocardial infarction(AMI) is known as heart attack. MI occurs when blood stops flowing properly to a part of the heart. MI occurred when one of the coronary arteries develops a blockage due to an unstable buildup of with blood, cholesterol and fat. This study aimed to determine the time interval between theonset ofclinical symptoms and receiving streptokinase inpatients withacutemyocardial infarction (AMI) at Amir Hospital in Zabol city in 2013. This was a cross-sectional-descriptive study. In total 72 patients were selected from all patients with chest pain and who referred to the emergency of Amir Hospital during 2013 in Zabol located in southeast of Iran. All patients were diagnosed with AMI. The instrument usedfordata collectionwas a questionnaire includingdemographic characteristics, record of exact location of infarction,ECG, the time of patients’ arrival to the emergency department (ED), and the time of receiving streptokinase.According to the obtained results of this study, the mean ofonset to call, call to door, anddoor to needletimes were 269.29±197.33, 34.02±12.32, and 96.33±49.95, respectively. The comparison ofonset to call, andcall to door and age, education and place of residence variables showed that there was a significant differencebetweenthese variables. In addition, the comparison ofdoor to needletime and age, sex, education and place of residence variables showed that there was no significant differencebetweenthese variables.Delay intreatmentof patients with STEMIiscaused as a result ofseveral factorsincluding rate of patient’sawarenessandemergency status. We need to provide opportunities to treatment ofMI by emergency and outpatientcenters. To reducing in-hospital delay, we must teach physiciansandemergency personnelto rapid diagnosis ofMI.