Fasciolosis is emerging as a serious medical and veterinary problem. Etiological agent for this economically important zoonotic disease are Fasciola hepatica and Fasciola gigantica which coexist in bovine hosts reared in Kashmir province because of overlap in the boundaries of their respective intermediate snail hosts. But there is predominance of Fasciola gigantica which incur heavy losses in bovine rearing industry. Present investigation was carried out for detection of Fasciola gigantica soluble antigens readily diagnosed by Fasciola gigantica infected bovine sera using Western blot analysis.