Evaluation of insulin like protein from dolichos lablab linn

Abstract

Leguminous plants are outstanding for their high levels of bioactive compounds, which can impact glucose metabolism by various mechanisms. Antidiabetic activity of leguminous plants is due to bioactive compounds such as genistein and daidzein, alpha-amylase inhibitors, alpha- glucosidase inhibitors. Present work focuses on assessment of edible bean, Dolichos lablab L. for the presence of insulin like proteins (ILP). Extraction of ILP was carried out by a modified, less laborious protocol based on fractional solubilization from germinating seeds of D. lablab L. Extracted ILP was purified by Reverse phase HPLC and collected fraction was assessed by SDS PAGE. The molecular weight was found to be 6KD. Further the in vitro assessment of the ILP was carried out using glucose uptake by yeast cells. Based on present work, it's evident that there are high chances of having insulin like proteins having antidiabetic potential in D.lablab L.