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A simple and low cost mitigation of extended-spectrum beta-lactamase producing pathogenic bacteria causing urinary tract infections using juglans regia extracts

Authors:Aashaq Hussain Allaie, Junaid Ahmad Ahangar, Kownsar Jan, Amrit Kour and R.P.Mishra
Int J Biol Med Res. 2023; 14(2): 7582-7585  |  PDF File  |  DOI No.: 11.2023-49453577

Abstract

The aim of the study was to evaluate the antibacterial evaluation of root extracts of Juglans regia against Extended Spectrum Beta Lactamase (ESBL) producing E. coli and Klebsiella pneumonia in Bombay Hospital and Research Centre Jabalpur. The antibacterial activity of, ethyl acetate and methanol root extracts of Juglans regia was determined by disk diffusion method. The antibacterial activity was calculated based on the minimum inhibitory concentration using Mueller–Hinton broth in a tube-dilution method. The best antibacterial activity, calculated as minimum inhibitory concentration values, against ESBL was shown by the methanol root extract Juglans regia (25 mg/mL) for both isolated organisms and ethyl acetate (25mg/mL) against E. coli. The methanol extract showed zone of inhibition in the range of 17-26mm as compared to ethyl acetate extract which showed zone of inhibition in the range of 11-16mm against the uropathogens. The zone of inhibition ranged from 17 mm to 26 mm and MIC was 25mg/ml. This effect is comparable to antibiotics. The results obtained in the present study suggest that Juglans regia have the potential to be developed as antibacterial agents against ESBL producing UTI bacteria strain. Further investigations are needed to identify the active compounds and their mechanism of action