The risk of infection in burns is well-known. In recent decades, the antimicrobial resistance of bacteria isolated from burn patients has increased. For this reason, a retrospective study was conducted at Father Muller hospital to analyze the bacterial isolates from the wounds of patients admitted to the Burn Unit and to determine the susceptibility patterns of the commonly cultured organisms over a 3-year period, January 2011 to December 2013. A total of 230 microorganisms were isolated from burn wounds of 179 patients. Our results revealed that the most frequent isolate was Acinetobacter baumannii (34%), Pseudomonas aeruginosa (29%), Klebsiella pneumonia (22%), Staphylococcus aureus (8%),Enterococcus spp (4%),Escherichia coli (1%). Multidrug-resistance has emerged as an important concern in our burn unit. Tigecycline, and colistin were found to be the most active drugs against Acinetobacter baumannii. Carbapenems and amikacin, were found to be the most active drugs against other gram negative bacteria. Vancomycin and linezolid were active against gram positive bacteria. Aggressive infection control measures, judicious use of antibiotics as per the prevailing antibiotic susceptibility patterns should be applied to limit the emergence and spread of multidrug-resistant pathogens.