In vitro study on total phenolics and antioxidant activities of the fruits crude extracts of annona squamosa collected from al jabal al akhdar, sultanate of oman

Abstract

Abstract : The major sources of natural antioxidants are plants and plant products. Medicinal plants may have different physiological action on human health. Natural antioxidants are reduce the risk of different illnesses such as cardiovascular diseases, intestinal cancer and infections symptoms. The aim of this work was to prepare different crude extracts from the fruits of Annona squamosa (AS) and evaluate their antioxidant activity and total phenolics content of the selected plant species collected from Jabal Al Akhdar, Sultanate of Oman. Soxhlet extraction method was used to prepare the crude extract from the fruits powder samples of the selected plant species with methanol. The prepared crude extract was completely defatted with water. Then it was extracted individually with different polarities of solvents with increasing polarity such as hexane, chloroform, ethyl acetate and butanol. The prepared different fruits crude extracts of AS were used for the determination of total phenolics content through Folin-Ciocalteu reagent method (FCR). Similarly, all fruits crude extracts from AS were used for the evaluation of antioxidant activity by using 2,2-Diphenyl-1-picrylhydrazyl method (DPPH). The results of total phenolics content in the fruits crude extracts were in the range of 76.5 to 635.75 mg/100 gm of dry powder samples. The highest antioxidant activity of different polarities crude extracts isolated from the fruits of AS was in hexane crude extract and lowest was chloroform crude extract and the order was found hexane>methanol >butanol> water >ethyl acetate>chloroform crude extract. In conclusion, isolated all crude extracts from the fruits of AS showed significant amount of total phenolics and strong antioxidant activity. Therefore, it could be used as a medicine for the treatment of different diseases.