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Production of rosmarinic in ocimum sanctum cell cultures by the influence of sucrose, phenylalanine, yeast extract, and methyl jasmonate

Authors:Lukmanul Hakkim F, Sivan kalyani, Mohammed Essa, S. Girija, Hyuk Song
Int J Biol Med Res. 2011; 2(4): 1070 -1074  |  PDF File


In this study, novel Ocimum sanctum in vitro cell suspension cultures were used to monitor the effects of different concentrations of sucrose (3.0, 4.0, 5.0, or 7.0%), phenylalanine (0.1, 0.25, 0.5, or 1.0 g/L) or elicitors including yeast extract (YE) (0.1, 0.25, 0.5, or 1.0 g/L) and methyl jasmonate (MJ) (100, 200, 300, or 500 M), on cell growth and rosmarinic acid (RA) production. The cellular content of RA increased slowly and reached the maximum after 18 days of inoculation, irrespective of treatment. An addition of sucrose at 5.0% showed considerable (2.7 fold) RA accumulation compare with control (3.0%) than other concentrations tested. Supplementation of phenylalanine at 0.25 g/L resulted in a higher accumulation of RA (4.1 fold) compared to non supplemented cells (RA content 5.7 mg/L/day; dry weight 0.98 g/L/day) than the other concentrations tested. Addition of YE at 0.5 g/L elevated RA content up to 7.0 fold of that found in non elicited suspension cells. Furthermore, a dramatic increase in cell growth and RA production was observed with MJ at 100 M. By overall MJ elicited cells were able to accumulate up to 8.6 folds of RA compared to non elicited cells. RA formation was paralleled with cell growth and it was isolated from suspension harvested biomass and characterized by spectral analysis.