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Evaluation of the effect of tigogenin on the activities of certain key enzymes of carbohydrate metabolism in streptozotocin induced diabetic rats

Authors:K Sunitha Kumari , G Immanuel , BS Dhanya , Abhila Raj
Int J Biol Med Res. 2012; 3(1): 1242-1247  |  PDF File

Abstract

This study was carried out to evaluate the role of key enzymes of carbohydrate metabolism in the antidiabetic action of Tigogenin. The Streptozotocin induced diabetic rats were treated with tigogenin at a dose of 10, 20 and 30 mg/kg body mass for 15 days. After 15 days, the blood samples were collected from each group of rats and the blood glucose level was estimated. The liver samples of rats were subjected to estimate the glycogen content, metabolic enzymes such as glycogen phosphorylase, glucose-6-phosphatase, fructose-1, 6 bisphosphatase and Hexokinase. A significant increase in blood glucose level was observed in diabetic rats. After treatment with tigogenin, the blood glucose level was found to be normal. Glycogen phosphorylase activity was greatly decreased in tigogenin treated rats. Similarly glucose-6-phosphatase and fructose-1,6 bisphosphatase activities were found to be reduced in tigogenin treated rats. In diabetic rats, the amount of glycogen, and Hexokinase activities were decreased, but they were normal in the tigogenin treated rats. The study indicates that tigogenin shows potent antihyperglycemic activity by the modulatory effect of key enzymes of carbohydrate metabolism and it is more pronounced when the dose of tigogenin is at 30mg/kg body weight.