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Seroprevalence of malaria by using dipstick method (malarigen kit) in the tertiary care hospital

Authors:Basavaraj K N, Rajendra Prasad BPM , Veena Manjunath , G. Vishwanath , Sai Priya B
Int J Biol Med Res. 2012; 3(1): 1356-1358  |  PDF File

Abstract

Background and objective: Microscopic examination of blood smears for malaria parasite is laborious , time consuming and requires skilled operators. Immunochromatographic dip stick assays provide a potential alternative . One such dipstick method (Malarigen kit) assay is based on detection of the Plasmodium intracellular metabolic enzyme lactate dehydrogenase(LDH). The differentiation of malarial parasites is based on the antigenic difference between the Plasmodium lactate dehydrogenase (pLDH ) isoforms. The rapid and specific diagnostic tests to identify individuals infected with malaria is paramount importance in efforts to control public health impact. Therefore, the test is valuable in emergency for rapid diagnosis of malaria. A study was therefore undertaken to find out the seroprevalence of malaria by using dipstick method. Material Methods: This study was conducted in the Department of Microbiology, J.J.M. Medical College,Davangre. Patients of both sexes and of all age groups with clinically suspected from September 2006 to November 2011 malaria were studied. Venous blood was collected by venipuncture into EDTA tubes for antigen detection. Results: Totally 4607 patients were screened, who were clinically suspected malaria, 2407 were males and 2200 were females with all age groups. A total of 492 cases were positive for malaria with incidence 10.67%.The P.falciparum were 272(55.3%) and P.vivax were 220(44.7%). Among 492 positive cases, males were 272 (55.3%), females were 220(44.7 %). In our study, the cases were more during rainy season 280(57%) between July to October. Conclusion: The rapid dipstick method (Malarigen kit) is a simple, sensitive, rapid test (10-15 minutes), ease of performance, interpretation, can be useful tool for the detection of Plasmodium falciparum and Plasmodium vivax where the laboratory support is limited in the rural areas.