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Isolation and nitrogen fixing efficiency of a novel endophytic diazotroph gluconacetobacter diazotrophicus associated with saccharum oficinarum from southern districts of tamilnadu

Authors:Murugan Meenakshisundaram, Karrupagnaniar Santhaguru
Int J Biol Med Res. 2010; 1(4): 298-300  |  PDF File


Gluconacetobacter diazotrophicus is a efficient nitrogen fixing diazotrophic bacterium is known to colonize Saccharum officinarum and fixes appreciable amount of atmospheric nitrogen. In the present study, Twenty four isolates of G.diazotrophicus were isolated from sugarcane (Root, stem and leaves) and designated as SoL1, SoL2, SoL3, SoL4, SoL5, SoL6, SoS1, SoS2, SoS3, SoS4, SoS5, SoS7, SoS8, SoS9, SoS10, SoS11, SoS12, SoS13, SoS14, SoS15, SoS16, SoR1, SoR2, SoR3 respectively. In the present investigation all the isolates showed appreciable amount of nitrogenase activity and the strain isolated from sugarcane stem (SoS2) showed ARA of maximum 410.92 nmoles of C2H4/hr mg cell protein followed by SoL3 from sugarcane leaf to the amount of 400.17 nmoles of C2H4/hr/mg cell protein and other strains were intermediate in their nitrogen fixation and hence the strain from sugarcane stem recommended as a effective biofertilizers.